p-Coumaroyl-meso-tartaric acid from spinach chloroplast preparations.
نویسندگان
چکیده
In a recent publication1 we reported on the isolation and identification of flavonoids and cinnamic acid derivatives from spinach chloroplast preparations. These cinnamic acid derivatives and flavonoids are obtained as low molecular fractions I and II from a water extract — called S^eth — from ether treated lyophilized chloroplasts, which is assumed to contain functional components of the photosynthetic electron transport chain between photosystem I and ferredoxin 2 '3 . We have discussed the possibility that a phenolic compound might be the prosthetic group of this functional component (possibly the primary acceptor) and also the relation of our phenolic compounds to other fractions of various activity isolated from chloroplasts by other authors 1. We have shown that the chromophoric group of low molecular fraction I consists of two esters of p-coumaric acid 1. The chemical nature of the compounds esterified to p-coumaric acid remained to be elucidated. We wish to report here on their identification. The p-coumaric acid derivatives of low molecular fraction I, absorbing at 312 nm, were obtained from the water extract of ether treated lyophilized chloroplasts as previously described After alkaline hydrolysis of its chromophore, the resultant alkaline solution was passed through a Dowex 50 WX 8 column and the p-coumaric acid extracted with ether1. The compound, which was originally esterified to p-coumaric acid, could now be found in the water phase. It was identified as meso-tartaric acid by paper chro-
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Photosynthetic cytochrome c reduction by isolated chloroplasts is mediated by substances with characteristics of a “Cytochrome c Reducing Substance” (CRS). A water soluble extract of ether treated, lyophilized chloroplasts (called SL-eth), related to the primary acceptor complex of photo system I, has CRS-activity. It contains p-coumaroyl-meso-tartaric acid. By comparison with model substances...
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ورودعنوان ژورنال:
- Zeitschrift fur Naturforschung. Teil B. Anorganische Chemie, organische Chemie, Biochemie, Biophysik, Biologie
دوره 27 5 شماره
صفحات -
تاریخ انتشار 1972